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dc.contributor.authorBordeaux, John Michael
dc.date.accessioned2014-03-04T03:24:30Z
dc.date.available2014-03-04T03:24:30Z
dc.date.issued2008-08
dc.identifier.otherbordeaux_john_m_200808_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/bordeaux_john_m_200808_ms
dc.identifier.urihttp://hdl.handle.net/10724/24835
dc.description.abstractAmylostereum areolatum is a wood-degrading fungus vectored by the European woodwasp Sirex noctilio recently introduced into North America. Together they constitute a pathosystem capable of killing healthy conifers, particularly pine. The fungus kills liquid cultured cells of loblolly pine and can infect living seedlings. The fungus can be cultured on a described defined medium. Amylostereum areolatum produces a ca. 75 kDa laccase (multicopper oxidase) and conditions have been established for maximizing laccase production over 11 days. A three-step purification yields a single laccase activity band. The laccase is likely monomeric and oxidizes ABTS, catechol, 1, 8-diaminonaphthalene, guaiacol, hydroquinone, phloroglucinol, p-phenylenediamine, and syringaldazine. Its oxidation of resveratrol indicates a possible role in detoxification. Laccase activity is maximum at pH 4.0 and 30°C. It is apparently inhibited by L-cysteine, sodium azide and sodium chloride. SDS and EDTA activate the enzyme. Sodium azide has been demonstrated as a true inhibitor.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectAmylostereum areolatum
dc.subjectSirex noctilio
dc.subjectPinus taeda
dc.subjectlaccase
dc.subjectphenoloxidase
dc.subjectresveratrol
dc.titleCharacterization of growth conditions for production of a laccase-like phenoloxidase by Amylostereum areolatum, a fungal pathogen of pines and other conifers
dc.typeThesis
dc.description.degreeMS
dc.description.departmentForest Resources
dc.description.majorForest Resources
dc.description.advisorJeffrey F. D. Dean
dc.description.committeeJeffrey F. D. Dean
dc.description.committeeScott Gold
dc.description.committeeClaiborne V. C. Glover, III


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