• Login
    View Item 
    •   Athenaeum Home
    • University of Georgia Theses and Dissertations
    • University of Georgia Theses and Dissertations
    • View Item
    •   Athenaeum Home
    • University of Georgia Theses and Dissertations
    • University of Georgia Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Development of high-throughput gene knockout strategies in Trypanosoma cruzi for production of attenuated lines

    Thumbnail
    Date
    2007-12
    Author
    Xu, Dan
    Metadata
    Show full item record
    Abstract
    In contrast to the substantial in silico studies of the T. cruzi genome, only a few genes have been experimentally characterized, mainly due to the lack of convenient methods for gene manipulation for reverse genetics studies. In this study we have compared the conventional multi-step cloning technique with two knockout strategies that have been proven to work in other organisms, One-Step-PCR- and Multisite Gateway-based systems. Our results show that while the One-Step-PCR strategy is faster than the other methods, it does not efficiently target genes of interest. The Multisite Gateway based approach, although not as fast and easy as the One-Step-PCR strategy, is less time-consuming than the conventional method and is able to efficiently delete target genes. Using this strategy, we have successfully generated single- and double- gene knockout parasites with genes that are predicted to be non-essential for epimastigote survival but essential for growth of T. cruzi amastigotes.
    URI
    http://purl.galileo.usg.edu/uga_etd/xu_dan_200712_ms
    http://hdl.handle.net/10724/24532
    Collections
    • University of Georgia Theses and Dissertations

    About Athenaeum | Contact Us | Send Feedback
     

     

    Browse

    All of AthenaeumCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    About Athenaeum | Contact Us | Send Feedback