• Login
    View Item 
    •   Athenaeum Home
    • University of Georgia Theses and Dissertations
    • University of Georgia Theses and Dissertations
    • View Item
    •   Athenaeum Home
    • University of Georgia Theses and Dissertations
    • University of Georgia Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Functional characterization of heterologously expressed Plasmodium falciparum ebl proteins

    Thumbnail
    Date
    2007-12
    Author
    Stokes, John Vincent
    Metadata
    Show full item record
    Abstract
    While heterologous expression has replaced biochemical purification as the principle method for obtaining purified, authentic proteins for characterization, no single expression system has proven appropriate for all applications. Heterologous expression of Plasmodium falciparum genes has proven especially challenging due to their extreme A+T codon bias and inappropriate post-translational modifications to product made by existing expression systems. These issues have generated interest in developing a novel heterologous expression system for P. falciparum. Here, we have transfected the ciliate Tetrahymena thermophila with a novel secretion vector bearing the R2 binding region of the P. falciparum genes, ebl-1 and jsebl. In addition, we demonstrated successful expression of the R2 region. This is the first demonstration of secreted P. falciparum antigens expressed by T. thermophila. We believe T. thermophila shows promise as a heterologous expression system for P. falciparum genes.
    URI
    http://purl.galileo.usg.edu/uga_etd/stokes_john_v_200712_ms
    http://hdl.handle.net/10724/24506
    Collections
    • University of Georgia Theses and Dissertations

    About Athenaeum | Contact Us | Send Feedback
     

     

    Browse

    All of AthenaeumCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    About Athenaeum | Contact Us | Send Feedback