• Login
    View Item 
    •   Athenaeum Home
    • University of Georgia Theses and Dissertations
    • University of Georgia Theses and Dissertations
    • View Item
    •   Athenaeum Home
    • University of Georgia Theses and Dissertations
    • University of Georgia Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Production of singleton proteins (expression, purification and crystallization) for structural genomics

    Thumbnail
    Date
    2007-12
    Author
    Ramiah, Annapoorani
    Metadata
    Show full item record
    Abstract
    The main objective of this thesis was to prepare samples (soluble proteins and crystals) of selected ORFan proteins for structure determination by X-ray crystallography. ORFan proteins are the proteins having no homologous sequence with any other proteins in other known organisms. Hence for ORFan proteins, structure prediction is not reliable and the only possible way to identify the protein family, fold is the determination of their three-dimensional structures. In this work, a select set of ORFan proteins from Pyrococcus furiosus (ORF PF0772), Aeropyrum pernix K1 (ORFs AP0305, AP0436, AP0371) and Clostridium thermocellum and (ORF Cthe_3042) have been expressed, purified and crystallized. Several crystallization techniques were employed to improve the quality of the diffraction of the crystals. Both seleneo-methionyl (Se-Met) incorporation and heavy atom soaking were used to provide initial phase information. Diffraction quality crystals were obtained for AP0305, AP0436, AP0371 and Cthe_3042. The structure of Cthe_3042 has been determined to 2.1Å resolution using Se-Met labeled protein.
    URI
    http://purl.galileo.usg.edu/uga_etd/ramiah_annapoorani_200712_ms
    http://hdl.handle.net/10724/24473
    Collections
    • University of Georgia Theses and Dissertations

    About Athenaeum | Contact Us | Send Feedback
     

     

    Browse

    All of AthenaeumCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    About Athenaeum | Contact Us | Send Feedback