• Login
    View Item 
    •   Athenaeum Home
    • University of Georgia Theses and Dissertations
    • University of Georgia Theses and Dissertations
    • View Item
    •   Athenaeum Home
    • University of Georgia Theses and Dissertations
    • University of Georgia Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Integron mediated antibiotic resistance genes in Shiga toxin-producing Escherichia coli and their transfer

    Thumbnail
    Date
    2007-12
    Author
    Nagachinta, Supakana
    Metadata
    Show full item record
    Abstract
    Approximately 7.9 and 0.6% of the Shiga toxin-producing Escherichia coli (STEC) isolates in our laboratory collection were positive for class 1 and 2 integron, respectively. These positive isolates were resistant to multiple antibiotics. However, only some of the antibiotic resistance traits were mediated by the integrons. A single gene cassette of aadA1 was identified on the class 1 integron and three gene cassettes, dfrA1, sat1, and aadA1, were found on the class 2 integron. The class 1 integron mediated antibiotic resistance genes were transferable to E. coli MG1655 during conjugation in nutrient broth. The transconjugants expressed the antibiotic resistance traits encoded by integron mediated, as well as non-integron mediated antibiotic resistance genes. Transfer of class 1 integron occurred in samples usually found in farm environments such as bovine feces and storm water. Environmental temperature and sample pH influenced the transfer frequencies of the integron mediated antibiotic resistance genes.
    URI
    http://purl.galileo.usg.edu/uga_etd/nagachinta_supakana_200712_ms
    http://hdl.handle.net/10724/24455
    Collections
    • University of Georgia Theses and Dissertations

    About Athenaeum | Contact Us | Send Feedback
     

     

    Browse

    All of AthenaeumCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    About Athenaeum | Contact Us | Send Feedback