Discovery of new regulatory transcription factors in Pyrococcus furiosus in metal stress and cold shock

Abstract

All living cells need to adapt to stress conditions in their environment. Under stress, the cells' primary response is at the level of transcription. This response may involve the binding of regulatory transcription factors to specific DNA sequences near the promoters or transcription initiation sites. Experiments have been done to retrieve the regulatory transcription factors in the model prokaryote, Pyrococcus furiosus, under metal (Fe) deficiency and cold shock. A putative regulatory transcription factor PF0250p has been found and binding was verified.