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dc.contributor.authorVunnava, Aswani
dc.date.accessioned2014-03-04T02:35:33Z
dc.date.available2014-03-04T02:35:33Z
dc.date.issued2007-05
dc.identifier.othervunnava_aswani_200705_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/vunnava_aswani_200705_ms
dc.identifier.urihttp://hdl.handle.net/10724/24026
dc.description.abstractBotulinum neurotoxin/A cleaves soluble N-ethyl Maleamide sensitive attachment receptor protein (SNAP-25) at the Neuro Muscular Junction, an important molecular protein in exocytosis and neurotransmitter release. This cleavage results in the blockade of the neurotransmission thus causing the flaccid muscle paralysis. Even though, heterogeneous spinal cord cultures provide the sensitive invitro model system to study the mechanism of action, the presence of the other population of cells in them, makes these mixed cultures not very pragmatic for the use of proteomics. The current study aims at addressing this issue of heterogeneity. In the present study motor neurons were enriched from the mixed spinal cord cell populations. Further, these enriched populations both quantitatively and qualitatively assessed for the motor neuron populations. Also, these enriched cultures were tested for the toxin sensitivity at different ages
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectBotlinum neurotoxin/A
dc.subjectNeuromuscular junction
dc.subjectMotor neurons
dc.subjectSNAP-25
dc.titleIn vitro analysis of enriched spinalcord cultures and their sensitivity to botulinum Neurotoxin-A
dc.typeThesis
dc.description.degreeMS
dc.description.departmentPhysiology and Pharmacology
dc.description.majorToxicology
dc.description.advisorJulie A. Coffield
dc.description.committeeJulie A. Coffield
dc.description.committeeMary Alice Smith
dc.description.committeeGaylen L. Edwards


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