Molecular detection and characterization of Infectious Bursal disease virus and chicken anemia virus from commercial broilers
Purvis, Linda Ann Brothers
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® Flinders Technology Associates filter paper (FTA card) was compared to phenol as a inactivation method for molecular detection and characterization of Infectious Bursal disease (IBD) using RT-PCR and nucleotide sequencing. After RT-PCR and nucleotide sequencing, the ®FTA card proved to be a reliable alternative to phenol for identification of IBDV. Chicken anemia virus (CAV) and IBDV are two of the most common immunosuppressive viruses associated with gangrenous dermatitis. Broiler farms with reoccurring gangrenous dermatitis were compared to farms identified as not experiencing gangrenous dermatitis. Molecular analysis of IBDV and CAV revealed that molecular changes are taking place. The isolates from a specific farm identified as MM were chosen for further characterization in vivo. Specific pathogen free and broiler birds inoculated with the MM isolates showed lymphocyte depletion indicating immunosuppression, which was seen in both groups of birds, indicating that these isolates could cause immunosuppression in the presence of maternal antibodies.