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dc.contributor.authorPurdee, Michaelle
dc.date.accessioned2014-03-04T01:11:37Z
dc.date.available2014-03-04T01:11:37Z
dc.date.issued2006-08
dc.identifier.otherpurdee_michaelle_l_200608_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/purdee_michaelle_l_200608_ms
dc.identifier.urihttp://hdl.handle.net/10724/23496
dc.description.abstractThe hThy28 gene was isolated from the human cervical carcinoma cell line, HeLa, and its expression was analyzed in these cells. Structural motif analysis reveals that hThy28 is a 225 amino acid protein that contains potential phosphorylation sites, a myristolation site, an amidation site, a glycosolation site, a sumoylation site, a DUF589 motif, and a nuclear localization site. Transfection of hThy28/Enhanced Green Fluorescent Protein constructs into HeLa cells revealed intense fluorescence in the nuclear localization of this gene product. Functional analysis of hThy28 was investigated using RNA interference methodology. These studies suggest that this gene may protect cells from undergoing apoptosis since hThy28 gene silencing promotes cell death in the presence of apoptotic mediators. Future work will focus on the role of hThy28 in the apoptotic pathway.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectApoptosis
dc.subjectRNA interference
dc.subjectThy28
dc.subjectHeLa cells
dc.subjectEnhanced Green Fluorescent Protein
dc.titleAnalysis of Thy28 expression in HeLa cells
dc.typeThesis
dc.description.degreeMS
dc.description.departmentPoultry Science
dc.description.majorPoultry Science
dc.description.advisorMark Compton
dc.description.committeeMark Compton
dc.description.committeeAdam Davis
dc.description.committeeAmy Batal


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