Analysis of Thy28 expression in HeLa cells
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The hThy28 gene was isolated from the human cervical carcinoma cell line, HeLa, and its expression was analyzed in these cells. Structural motif analysis reveals that hThy28 is a 225 amino acid protein that contains potential phosphorylation sites, a myristolation site, an amidation site, a glycosolation site, a sumoylation site, a DUF589 motif, and a nuclear localization site. Transfection of hThy28/Enhanced Green Fluorescent Protein constructs into HeLa cells revealed intense fluorescence in the nuclear localization of this gene product. Functional analysis of hThy28 was investigated using RNA interference methodology. These studies suggest that this gene may protect cells from undergoing apoptosis since hThy28 gene silencing promotes cell death in the presence of apoptotic mediators. Future work will focus on the role of hThy28 in the apoptotic pathway.