Leukocyte production of reactive oxygen species, and circulating concentrations of hydrogen peroxide and glutathione in healthy horses and horses with gastrointestinal disease
Goan, Elpinikki E.
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The first goal of this project was to characterize the inflammatory state of healthy horses. The average endogenous and stimulated production of reactive oxygen species for 80 horses was 3.03 ± 0.26 and 259.20 ± 8.87 average fluorescent units (AFU), respectively. Average plasma levels of hydrogen peroxide for 54 healthy horses were 0.41 ± 0.03 OD, and reduced (GSH) and oxidized (GSSG) glutathione for 41 horses averaged 641.3 ± 53.5 and 13.5 ± 2.5 M, respectively. The mean GSH/GSSG ratio was 147.1 ± 30.0. An overall stress index was calculated as the product of endogenous reactive oxygen species production and total leukocyte count. The same parameters were measured in 62 horses with gastrointestinal diseases, and were compared with method of treatment (medical or surgical) and outcome (survived or died). The results obtained suggest that GSH/GSSG ratio, plasma level of hydrogen peroxide and the overall stress index may help distinguish horses requiring medical or surgical treatment, and provide information regarding prognosis. Key Words: Reactive oxygen species, neutrophils, horse, oxidant, antioxidant, gastrointestinal disease