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dc.contributor.authorZhang, Shu
dc.date.accessioned2014-03-04T01:07:43Z
dc.date.available2014-03-04T01:07:43Z
dc.date.issued2006-05
dc.identifier.otherzhang_shu_200605_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/zhang_shu_200605_ms
dc.identifier.urihttp://hdl.handle.net/10724/23296
dc.description.abstractPiCyt1 is a protein which was identified from the hemocytes of Pseudoplusia includens (Lepidoptera: Noctuidae). This protein is the antigen of 43E9A10, a monoclonal antibody which recognizes specifically one type of hemocyte named plasmatocytes. In this project, I studied some basic biological characteristics and functions of this protein. The biological characters include the encoding cDNA and protein sequences, the expression tissue, transcriptional pattern thin 5 instar P. includens larvae, the influence of some immune challenges on the transcription and secretion of this protein. Based on the facts that PiCyt1 shares homology with a hemolytic protein from Aspergillus fumigatus, Asp-hemolysin, we hypothesize that it has a similar function. Here I transiently expressed PiCyt1 in selected insect cell lines and purified the recombinant protein through the attached 6xHis tag. The cytolytic activity assay of recombinant PiCyt1 was conducted against both prokaryotic and eukaryotic targets.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectPlasmatocyte
dc.subjectPlasmatocyte Spreading Peptide (PSP)
dc.subjectPseudoplusia include
dc.subjectpIZT/V5-His vector
dc.subjectcytolytic activity
dc.titleBasic biological characters and functions of Pseudoplusia includens protein PicyT1
dc.typeThesis
dc.description.degreeMS
dc.description.departmentEntomology
dc.description.majorEntomology
dc.description.advisorMichael Strand
dc.description.committeeMichael Strand
dc.description.committeeMichael Adang
dc.description.committeeKevin Clark


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