Investigation of the chemical nature of alkaline scouring and the effect on seed coat fragments
MetadataShow full item record
The biomass of cotton seed coat fragments (SCFs) was investigated using CE-MS, HPLC-UVD and HPLC-RID. The main compositions of SCFs were found to be water-soluble materials (7.55%), structural carbohydrates (30.12%), lignins (27.28%), pigments (17.67%), and other unknown residues (3.11%). The carbohydrate analysis with HPLC-RID identified two carbohydrate monomers in SCFs: D (+) glucose and D (+) xylose. A fatty acid with a molecular 3weight of 330 was found by CE-MS in the alkaline hydrolysis products and its CID-MS fragmentation was carried out to determine the possible structure. Lignin analysis identified several compounds with strong UV absorptions from 200 nm to 320 nm. These compounds were possibly phenols, guaiacols, vanillins and ferulic acids. It was also observed that all of the pigments, most of the xylose, and about 65 percent of lignin of SCFs were hydrolyzed in the alkali scouring. This result probably indicates that successful bioscouring requires similar chemical functions in breaking down SCFs. Finally, scanning electron microscopy (SEM) and light microscopy (LM) were used to acquire insight into the physical structures of the different layers of SCFs.