The effects of leptin, gip and clenbuterol on bone marrow gene expression
Lackay, Sarah Nichole
MetadataShow full item record
Administration of Leptin, GIP, and Clenbuterol results in increased adipogenesis and decreased osteoblastogenesis in mice and rats. Microfluidic technology was used to analyze changes in expression of 23 genes chosen for their association with adipocytes, osteoblasts, and varying growth factors. Comparison of injection location showed that there was no significant difference between LV, ARC and VMH injection of Leptin on gene expression in rats. Treatment with GIP decreased overall gene expression according to Tukey’s test (p<0.0001). However, varying doses of GIP elicited no different effect on gene expression, implying a threshold level of GIP that must be met to induce a decrease in gene expression. Leptin also decreased overall gene expression relative to control (p<0.0001). Gene expression in rats treated with Leptin was significantly lower than gene expression in rats treated with GIP 1.0 µg (p=0.0059) and GIP 10 µg (p=0.0131). This suggests that Leptin has a more potent effect on bone marrow than does GIP. Treatment with Clenbuterol also elicited a decreased expression of bone marrow genes (p<0.0001).