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dc.contributor.authorSon, Insook
dc.date.accessioned2014-03-03T23:28:37Z
dc.date.available2014-03-03T23:28:37Z
dc.date.issued2005-12
dc.identifier.otherson_insook_200512_phd
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/son_insook_200512_phd
dc.identifier.urihttp://hdl.handle.net/10724/23011
dc.description.abstractBroiler carcasses (n = 325) were sampled in a U.S. commercial poultry processing plant during five plant visits from August to October of 2004 at three sites along the processing line: 1) pre-scalding, 2) pre-chilling, and 3) post-chilling. Arcobacter species were recovered from pre-scalded carcasses more frequently (96.8%) than from pre-chilled (61.3%) and post-chilled carcasses (9.6%). For Arcobacter identification, a species-specific multiplex PCR assay showed that A. butzleri was the most prevalent species (79.1%) followed by A. cryaerophilus 1B (18.6%). A. cryaerophilus 1A was found at low levels (2.3%) and A. skirrowii was not isolated at all. Campylobacter was isolated from 92% of pre-scalded carcasses, 100% of pre-chilled carcasses, and 52% of post-chilled carcasses. For Campylobacter ®speciation, the BAX PCR identified as C. jejuni (87.6%) as the most common species followed by C. coli (12.4%). The genetic diversity of Arcobacter and Campylobacter was analyzed by pulsed field gel electrophoresis (PFGE). Genomic DNA was digested with KpnI from Arcobacter strains and SmaI from Campylobacter strains. A total of 32.8% of Arcobacter isolates belonged to single-isolate groups, while only 2.3% of Campylobacter isolates belonged to this category. The remaining Arcobacter species were distributed among 25 multi-isolate PFGE groups, while Campylobacter species were found in just eight multi-isolate groups. The great majority of Arcobacter (93.7%) and Campylobacter (99.5%) isolates were resistant to one or more antimicrobials. Multiple antimicrobial resistance was observed in 71.8% of the Arcobacter isolates and in 28.4% of the Campylobacter isolates. Of the A. butzleri isolates, 89.9% (n = 125) were resistant to clindamycin, 82% (n = 114) were resistance to azithromycin, and 23.7% (n = 33) were resistant to nalidixic acid. Resistance to tetracycline was very high in C. jejuni and C. coli at 99.5% and 96.3%, respectively. These data suggest significant contamination of Arcobacter and Campylobacter from carcasses from different processing sites in a commercial poultry plant with a high genetic diversity of Arcobacter, and demonstrated resistance in Arcobacter and Campylobacter to common antimicrobial agents.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectArcobacter
dc.subjectBroiler chickens
dc.subjectCampylobacter
dc.subjectPoultry processing
dc.subjectGenetic diversity
dc.subjectPFGE
dc.subjectTyping
dc.subjectAntimicrobial resistance
dc.titlePrevalence, genetic diversity, and antimicrobial resistance patterns of arcobacter and campylobacter on broiler carcasses during processing
dc.typeDissertation
dc.description.degreePhD
dc.description.departmentFood Science and Technology
dc.description.majorFood Science
dc.description.advisorMark A. Harrison
dc.description.committeeMark A. Harrison
dc.description.committeePaula J. Fedorka-Cray
dc.description.committeeMark D. Englen
dc.description.committeeJoseph F. Frank
dc.description.committeeLarry R. Beuchat


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