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dc.contributor.authorRogers, Theresa Elizabeth
dc.date.accessioned2014-03-03T23:28:25Z
dc.date.available2014-03-03T23:28:25Z
dc.date.issued2005-12
dc.identifier.otherrogers_theresa_e_200512_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/rogers_theresa_e_200512_ms
dc.identifier.urihttp://hdl.handle.net/10724/22999
dc.description.abstractIn forested stream ecosystems of the north and eastern United States, larvae of the aquatic crane fly Tipula abdominalis are important shredders of leaf litter detritus. T. abdominalis larvae harbor a dense and diverse microbial community in their hindgut that may aide in the degradation of lignocellulose. In this study, the activities of cellulolytic and hemicellulolytic enzymes were demonstrated from hindgut extracts and from bacterial isolates using model sugar substrates. One of the bacterial isolates was characterized and represents a novel genus, Crocebacterium gen, nov., within the family Microbacteriaceae, and the type species is Crocebacterium ilecola gen, nov., sp. nov. A genomic library of this novel bacterium was constructed to find the genes that encode cellulases and hemicellulases the bacterium produces. A metagenomic library was also constructed in an attempt to find the genes encoding cellulases and hemicellulases from the T. abdominalis hindgut community, including as yet uncultivated microorganisms.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectCrocebacterium ilecola
dc.subjectTipula abdominalis
dc.subjectlignocellulose degradation
dc.subjectmetagenomic library
dc.subjectmethylumbelliferone
dc.titleCellulase and hemicellulase activity within the Tipula abdominalis larval gut
dc.typeThesis
dc.description.degreeMS
dc.description.departmentMicrobiology
dc.description.majorMicrobiology
dc.description.advisorJoy B. Peterson
dc.description.committeeJoy B. Peterson
dc.description.committeeEric Stabb
dc.description.committeeWilliam B. Whitman
dc.description.committeeJuergen Wiegel


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