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dc.contributor.authorWallace, Erica Renee
dc.date.accessioned2014-03-03T23:11:11Z
dc.date.available2014-03-03T23:11:11Z
dc.date.issued2004-12
dc.identifier.otherwallace_erica_r_200412_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/wallace_erica_r_200412_ms
dc.identifier.urihttp://hdl.handle.net/10724/22258
dc.description.abstractProteomics is the comprehensive and systematic study of the proteome, which is defined as the set of all expr essed proteins in a cell, tissue or organism. The standard method for quantitative proteome analysis combines protein separation by two -dimensional gel electrophoresis (2DGE) with mass spectrometric (MS) identification of selected protein spots. Because these techniques allow one to study the full protein complement of a cell or tissue at the time of isolation, they are beneficial for identifying the pool of proteins whose expression is related to a particular treatment and to each other. This paper will pr esent two proteomic studies of micronutrient metabolism. In the first study, the effect of zinc deficiency on protein expression in a colon adenocarcinoma (Caco -2) cell-culture model was investigated. In the second study, proteomics techniques were used to identify alterations in cellular protein expression, due to transfection with a selenoprotein construct ( pro-fs) in Madin-Darby Canine Kidney cells.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectTwo-dimensional gel electrophoresis
dc.subjectZinc
dc.subjectSelenium
dc.subjectCaco-2
dc.subjectMadin- Darby Canine Kidney
dc.subjectZinc-deficiency
dc.subjectpro-fs
dc.subjectHIV-1
dc.subjectproteomics
dc.subjectmass spectrometry
dc.titleUsing proteomics as a tool for the study of micronutrient metabolism
dc.typeThesis
dc.description.degreeMS
dc.description.departmentFoods and Nutrition
dc.description.majorFoods and Nutrition
dc.description.advisorArthur Grider
dc.description.committeeArthur Grider
dc.description.committeeEthan Will Taylor
dc.description.committeeJames Hargrove


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