Using proteomics as a tool for the study of micronutrient metabolism
Wallace, Erica Renee
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Proteomics is the comprehensive and systematic study of the proteome, which is defined as the set of all expr essed proteins in a cell, tissue or organism. The standard method for quantitative proteome analysis combines protein separation by two -dimensional gel electrophoresis (2DGE) with mass spectrometric (MS) identification of selected protein spots. Because these techniques allow one to study the full protein complement of a cell or tissue at the time of isolation, they are beneficial for identifying the pool of proteins whose expression is related to a particular treatment and to each other. This paper will pr esent two proteomic studies of micronutrient metabolism. In the first study, the effect of zinc deficiency on protein expression in a colon adenocarcinoma (Caco -2) cell-culture model was investigated. In the second study, proteomics techniques were used to identify alterations in cellular protein expression, due to transfection with a selenoprotein construct ( pro-fs) in Madin-Darby Canine Kidney cells.