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dc.contributor.authorWaldo, Robert Hendren
dc.date.accessioned2014-03-03T23:11:10Z
dc.date.available2014-03-03T23:11:10Z
dc.date.issued2004-12
dc.identifier.otherwaldo_robert_h_200412_phd
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/waldo_robert_h_200412_phd
dc.identifier.urihttp://hdl.handle.net/10724/22257
dc.description.abstractCytadherence or attachment to host tissue is a crucial event in the pathogenesis of infections by Mycoplasma pneumoniae, a common agent of respiratory disease in humans. A large group of cytadherence-accessory proteins work together to localize the major adhesin P1 to the polar attachment organelle in a functional form. The hmw gene cluster, which contains the genes encoding cytadherence-accessory proteins P30, HMW1, and HMW3 in addition to other possible cytadherence proteins, was found to be transcribed from four different promoters in a series of overlapping transcripts that may impact the regulation of genes in the cluster. In another cluster, the p1 operon, genes MPN140-142 were transcribed as polycistronic message. Recombinant genes of the p1 operon encoding the P1 adhesin and the proteins B and C were delivered and expressed in non-adherent mutants lacking some or all of these proteins. Some but not all translation of the gene encoding B/C (MPN142) was linked to the translation of the gene encoding P1 (MPN141). Furthermore, in the absence of P1, B/C were unstable and little or no protein was detected. This linkage of P1/B/C at transcription, translation, and protein stability sheds light on relationships between P1/B/C on the genetic and biochemical level and supports the view of these three proteins as integral members of a single major adhesin complex.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectMycoplasma pneumoniae
dc.subjectAtypical pneumonia
dc.subjectP1
dc.subjectA
dc.subjectB
dc.subjectC
dc.subjectP90
dc.subjectP40
dc.subjectHMW1
dc.subjectHMW3
dc.subjectP30
dc.subjectMutant I-2
dc.subjectMutant II-3
dc.subjectMutant III-4
dc.subjectMutant IV-22
dc.subjectBacteria
dc.subjectProkaryotes
dc.subjectAdhesins
dc.subjectCytadherence
dc.subjectCytadherence-accessory proteins
dc.subjectGenetics
dc.subjectGene regulation
dc.subjectPromoter
dc.subjectRNA
dc.subjectTranscription
dc.subjectTranslational coupling
dc.subjectOperon
dc.subjectGene cluster
dc.subjectProtein stability
dc.subjectComplementation
dc.subjectChloramphenicol acetyltransferase
dc.subjectReverse transcriptase-polymerase chain reaction
dc.subjectPrimer extension
dc.subjectWestern/Immuno-blot
dc.subjectRHemadsorption
dc.subjectImmuno-fluorescence microscopy.
dc.titleGenetic and biochemical analysis of cytadherence-related operons in Mycoplasma pneumoniae
dc.typeDissertation
dc.description.degreePhD
dc.description.departmentMicrobiology
dc.description.majorMicrobiology
dc.description.advisorDuncan C. Krause
dc.description.committeeDuncan C. Krause
dc.description.committeeHarry A. Dailey
dc.description.committeeMargie D. Lee
dc.description.committeeEllen L. Neidle
dc.description.committeeEric V. Stabb


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