Characterization of porcine trophectoderm derived cells to establish a model for placental development
Hodges, Kathryn Stewart
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A porcine trophoblastic cell line could provide a powerful model for understanding trophoblast cell biology as well as placental gene expression and proteomics in vitro. Bone morphogenetic protein 4 (BMP4) has been shown to induce differentiation of human embryonic stem cells into trophoblast lineages. In this experiment, elongated embryos were flushed from the hysterectomized uteri of superovulated and bred prepuberal gilts 15 days post insemination. The trophectoderm tissue was manually dissected into cell aggregates and plated on collagen Type IV, Matrigel, human extracellular matrix (laminin, collagen type IV and heparan sulfate proteoglycan derived from human placenta) or mouse embryonic fibroblast (MEF) feeder layers in a DMEM based culture medium in the presence or absence of BMP4. Cells were passaged and only cells growing on Matrigel or MEF feeder layers could be further cultured. These data suggest that both the cell substrate and BMP4 affect initial trophoblast outgrowths.