Role of curli fimbriae in mediating the cells of enterohemorrhagic Escherichia coli to attach to abiotic and biotic surfaces
PAWAR, DHARMENDRASINGH MAKHANSINGH
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Cells of enterohemorrhagic E. coli (EHEC) express long, thin, wiry fimbriae know as curli. Three pairs of EHEC cultures, each with a curli-expressing and non curli-expressing strain were used in this study to examine the effect of incubation temperature and growth media on curli-expression, and the role of curli in attachment to abiotic and biotic surfaces. Growth of the cells on 3 growth media at 5 incubation temperatures revealed that curli is not expressed at 10ºC, and curli phenotypic conversion took place under appropriate incubation conditions. Results of crystal violet binding assays indicate that cells of the curli-expressing strains attached better than did the cells of the non curli-expressing strains. The curli-expressing cells also attached more efficiently than the non curli-expressing cells on beef with the smallest differences of 0.12 and 0.22 Log10CFU/cm2 and the largest differences of 0.86 and 1.17 Log10CFU/cm2 on raw beef and beef salami, respectively.