Subnuclear localization and interaction of selected Aux/IAA and ARF proteins in vivo
Majsztrik, John Christopher
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The plant hormone auxin has been studied for many years and is instrumental for plant growth and development. Auxin regulates gene transcription of at least five families including the Aux/IAA family. Aux/IAA and ARF proteins are known to interact through conserved domains in both the Aux/IAA and ARF families. This research is the first to report individual nuclear and sub-nuclear localization patterns of the Arabidopsis ARF and Aux/IAA protein in vivo, focusing on ARF1 and IAA17. Several co-localizations of proteins from the same and different families exhibited protein recruitment to structures and locations individual proteins did not localize to when expressed alone. This work strongly supports the putative interactions between ARF and Aux/IAA proteins in vivo. Northern Blot and semi-quantitative RT PCR showed that ARF1 and IAA17 were co-expressed in tissues sampled. The role of putative nuclear localization signals was investigated by localization of deletion constructs of IAA17 and ARF1.