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dc.contributor.authorDebnam, Antoinette Lorraine
dc.date.accessioned2014-03-03T21:23:15Z
dc.date.available2014-03-03T21:23:15Z
dc.date.issued2004-08
dc.identifier.otherdebnam_antoinette_l_200408_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/debnam_antoinette_l_200408_ms
dc.identifier.urihttp://hdl.handle.net/10724/21801
dc.description.abstractEnterococci may be reservoirs of antibiotic resistance, and it is important to characterize the strains isolated from animals and their environments. This study analyzed enterococci from four poultry houses for six growouts on a farm. Two houses on the farm were control houses and did not use any antimicrobials while two other houses on each farm used flavomycin, virginiamycin, and bacitracin during different poultry growouts. Litter, chick boxliners, feed, and poultry carcasses were obtained from each house and cultured for the presence of enterococci. Enterococci species were identified using a species-specific multiplex-PCR. Vitek, a commercial culture typing system, was also used a confirmatory procedure. Additionally, Enterococcus faecium isolates were further characterized using BOX-PCR and Pulsed- Field Gel Electrophoresis (PFGE). Enterococcus faecalis (n=763; 52%) and E. faecium (n=578; 40%), were isolated most often from the farm and houses regardless of antimicrobial treatment. Enterococcus faecium analyzed by BOX-PCR and PFGE appeared to be genetically different as few clusters were observed.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectEnterococcus
dc.subjectPFGE
dc.subjectviriginiamycin
dc.subjectBOX PCR
dc.subjectpoultry
dc.titleMolecular analysis of Enterococcus spp. in a poultry setting
dc.typeThesis
dc.description.degreeMS
dc.description.departmentMedical Microbiology
dc.description.majorMedical Microbiology
dc.description.advisorDavid Suarez
dc.description.committeeDavid Suarez
dc.description.committeeCharles Hofacre
dc.description.committeeSusan Little


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