Adsorption of purified cellulases on cotton fibers
Kanchagar, Anand Purushottam
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Commercial cellulases are a mixture of endoglucanases (EGs), exoglucanases (CBHs) and glucosidases. EGs and CBHs differ in their action on cellulose as a virtue of their structure but are adsorbed on cellulose to conduct hydrolysis. The nature of cellulase adsorption on cellulose has been explained by adsorption models such as Langmuir model in the past. In the present investigation, Linear, Langmuir, Freundlich and combined Langmuir-Freundlich adsorption models were used to explain the adsorption of CBH I, II and EG II. CBH I, II and EG II were purified and adsorption of the purified enzymes on cotton fibers was collected. Statistical analysis was performed to determine the best fitting model. It was found that more than one adsorption model can be used to explain the adsorption of CBH I, II and EG II on cotton fibers in the given range of enzyme concentration indicating that the substrate is not saturated by the enzyme. The temperature of adsorption was found to affect adsorption of CBH I, II and EG II on cotton fibers to a small extent. Adsorption of CBH I and II was found to be higher at 55 ºC as compared to 25, 35 and 45 ºC. Effect of temperature on adsorption of EG II on cotton fibers was more pronounced at higher concentrations of the enzyme.