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dc.contributor.authorSimunovic, Vesna
dc.date.accessioned2014-03-03T20:22:11Z
dc.date.available2014-03-03T20:22:11Z
dc.date.issued2002-12
dc.identifier.othersimunovic_vesna_200212_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/simunovic_vesna_200212_ms
dc.identifier.urihttp://hdl.handle.net/10724/20672
dc.description.abstractThe inability to separate inner and outer membranes in wild-type Myxoccocus xanthus DK1622 has been a major obstacle in examining the physiology of this organism. Using spheroplasting in combination with sucrose density centrifugation we now report isolation and characterization of several membrane fractions. Biochemical analysis suggests that these fractions correspond to inner, hybrid and outer membranes. A fifth membrane fraction contains a Ta-1 polyketide synthetase. Two dimensional SDS-PAGE gel analysis indicated the presence of distinct inner and outer membrane proteomes. Using this approach in conjunction with Western blotting, we demonstrate differential localization of several surface-associated proteins required for social motility and signaling.
dc.languageeng
dc.publisheruga
dc.rightspublic
dc.subjectMembrane separation
dc.subjectSucrose density centrifugation,
dc.titleSeparation and characterization of membranes from vegetative, Myxoccocus xanthus DK1622 cells
dc.typeThesis
dc.description.degreeMS
dc.description.departmentMicrobiology
dc.description.majorMicrobiology
dc.description.advisorLawrence J. Shimkets
dc.description.committeeLawrence J. Shimkets
dc.description.committeeTimothy Hoover
dc.description.committeeEric Stabb


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