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dc.contributor.authorZhang, Weicheng
dc.date.accessioned2014-03-03T20:07:27Z
dc.date.available2014-03-03T20:07:27Z
dc.date.issued2001-12
dc.identifier.otherzhang_weicheng_200112_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/zhang_weicheng_200112_ms
dc.identifier.urihttp://hdl.handle.net/10724/20490
dc.description.abstractThe general purpose of this study was to confirm or rule out the possibility that microgravity and hypoxia were factors in the previously observed culture color change, from yellow to black/brown in differentiation of N1E-115 neuroblastoma cells cultured under simulated microgravity. In this study, N1E-115 cells were cultured and differentiated under microgravity on cytodex 3 beads in HARV bioreactor. Control cultures were differentiated under normal gravity in petri and tissue culture dishes. The culture vessels were operated in a CO2 incubator at different incubator oxygen levels of hypoxia (10%), normoxia (18%) and hyperoxia (26%). It was not possible to replicate color development by differentiating neuroblastoma cells under simulated microgravity suggesting that the phenomena previously observed in our laboratory and by other investigations was not solely dependent on microgravity, if at all. Metabolic differences (O2 consumption pattern) observed between cells differentiated under normal and microgravity were attributed more to O2 mass transport limitation into the HARV. Also, hopoxia was not proven to be a factor in transdifferentiation of N1E-115 neuroblastoma cells to melanocyte-like cells.
dc.publisheruga
dc.rightspublic
dc.subjectN1E-115 Neuroblastoma cell
dc.subjectcell differentiation
dc.subjectmicrogravity
dc.subjectHigh Aspect Rotation Vessel (HRAV)
dc.subjecthypoxia
dc.subjectnormoxia
dc.subjecthyperoxia
dc.subjectpigment cell
dc.subjecttyrosinase.
dc.titleGrowth and differentiation of N1E-115 neuroblastoma cells in High Aspect Rotating Vessel (HARV)under hypoxia, normoxia and hyperoxia
dc.typeThesis
dc.description.degreeMS
dc.description.departmentBiological and Agricultural Engineering
dc.description.majorBiological Engineering
dc.description.advisorWilliam S. Kisaalita
dc.description.committeeWilliam S. Kisaalita
dc.description.committeeCharles H. Keith
dc.description.committeeTimothy L. Foutz


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