Show simple item record

dc.contributor.authorBerman, Brion Allen
dc.date.accessioned2014-03-03T20:02:58Z
dc.date.available2014-03-03T20:02:58Z
dc.date.issued2001-12
dc.identifier.otherberman_brion_a_200112_ms
dc.identifier.urihttp://purl.galileo.usg.edu/uga_etd/berman_brion_a_200112_ms
dc.identifier.urihttp://hdl.handle.net/10724/20281
dc.description.abstractHemerythrin (Hr) is a non-heme iron protein, which originally had only been found in marine invertebrates where it functions as an oxygen-transport or storage protein. Hrs typically contain an oxo-/hydroxo-bridged non-heme diiron site surrounded by a four-helical bundle protein fold. All Hrs have seven conserved amino acids that furnish iron ligands, namely five histidines, one aspartate and one glutamate. Subsequently, homologues have been found in other organisms. Through protein and nucleotide searches, a previously undescribed protein having the Hr-like sequence motif (MjHr), was discovered in the Methanococcus jannaschii genome. MjHr exhibits structural and spectral properties resembling those of previously characterized Hrs. This Hr-like protein is the first to be described from archaea. Unlike Hrs that function as O2- carrying proteins, MjHr is proposed to function as an O2 sensor or scavenger within this methanogenic anaerobe.
dc.publisheruga
dc.rightspublic
dc.subjectHemerythrin
dc.subjectMethanococcus jannaschii
dc.subjectO2-binding
dc.subjectStopped-flow spectrophotometry
dc.subjectElectron paramagnetic resonance (EPR)
dc.subjectCircular dichroism (CD)
dc.subjectWestern-blot
dc.subjectMetalloprotein
dc.subjectReconstitution
dc.subjectOverexpression
dc.subjectProtein Purification
dc.titleStudies of a novel hemerythrin-like protein from the anaerobic, hyperthermophilic archaeon, methanococcus jannaschii
dc.typeThesis
dc.description.degreeMS
dc.description.departmentChemistry
dc.description.majorChemistry
dc.description.advisorDonald M. Kurtz, Jr.
dc.description.committeeDonald M. Kurtz, Jr.
dc.description.committeeRobert S. Phillips
dc.description.committeeMichael K. Johnson


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record