Targeting of exogenous proteins into the MHC class I antigen presentation pathway
MetadataShow full item record
CD8 + T cells are critical to immune control of Trypanosoma cruzi infection and targets of this response are potential vaccine candidates. In this study, we explored the utilization of HIV tat 49-57 (TAT peptide) to deliver potential CTL target proteins to the MHC class I antigen presentation pathway as a method to identify CD8 + T cell targets. Purified 'misfolded' chicken ovalbumin (OVA) fused with TAT, i.e. TAT-HA-OVA, was shown to translocate into the cytosol of the cells while HA-OVA was not. However, both TAT-HA-OVA and HA-OVA were processed and presented by MHC class I molecules for the recognition and activation of CD8 + T cells. In addition, purified TAT-HA-TSA-1 (Trypomastigote-surface-antigen-1) and HA-TSA-1 were able to activate TSA-1 specific CD8 + T cells as well. These results suggest that denatured fusion proteins, with or without TAT translocation sequence, are able to be processed and presented in association with MHC class I molecules.