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dc.contributor.authorHong, Yang
dc.contributor.authorLiu, Tongrui
dc.contributor.authorLee, Margie D
dc.contributor.authorHofacre, Charles L
dc.contributor.authorMaier, Marie
dc.contributor.authorWhite, David G
dc.contributor.authorAyers, Sherry
dc.contributor.authorWang, Lihua
dc.contributor.authorBerghaus, Roy
dc.contributor.authorMaurer, John J
dc.date.accessioned2013-06-12T15:15:09Z
dc.date.available2013-06-12T15:15:09Z
dc.date.issued2008-10-09
dc.identifier.citationBMC Microbiology. 2008 Oct 09;8(1):178
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2180-8-178
dc.identifier.urihttp://hdl.handle.net/10724/19748
dc.description.abstractAbstract Background Classical Salmonella serotyping is an expensive and time consuming process that requires implementing a battery of O and H antisera to detect 2,541 different Salmonella enterica serovars. For these reasons, we developed a rapid multiplex polymerase chain reaction (PCR)-based typing scheme to screen for the prevalent S. enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium. Results By analyzing the nucleotide sequences of the genes for O-antigen biosynthesis including wba operon and the central variable regions of the H1 and H2 flagellin genes in Salmonella, designated PCR primers for four multiplex PCR reactions were used to detect and differentiate Salmonella serogroups A/D1, B, C1, C2, or E1; H1 antigen types i, g, m, r or z10; and H2 antigen complexes, I: 1,2; 1,5; 1,6; 1,7 or II: e,n,x; e,n,z15. Through the detection of these antigen gene allele combinations, we were able to distinguish among S. enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium. The assays were useful in identifying Salmonella with O and H antigen gene alleles representing 43 distinct serovars. While the H2 multiplex could discriminate between unrelated H2 antigens, the PCR could not discern differences within the antigen complexes, 1,2; 1,5; 1,6; 1,7 or e,n,x; e,n,z15, requiring a final confirmatory PCR test in the final serovar reporting of S. enterica. Conclusion Multiplex PCR assays for detecting specific O and H antigen gene alleles can be a rapid and cost-effective alternative approach to classical serotyping for presumptive identification of S. enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium.
dc.titleRapid screening of Salmonella enterica serovars Enteritidis, Hadar, Heidelberg and Typhimurium using a serologically-correlative allelotyping PCR targeting the O and H antigen alleles
dc.typeJournal Article
dc.date.updated2013-06-07T18:44:23Z
dc.description.versionPeer Reviewed
dc.language.rfc3066en
dc.rights.holderYang Hong et al.; licensee BioMed Central Ltd.


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