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dc.contributor.authorMenendez, Laura
dc.contributor.authorWalker, L DeEtte
dc.contributor.authorMatyunina, Lilya V
dc.contributor.authorTotten, Kimberly A
dc.contributor.authorBenigno, Benedict B
dc.contributor.authorMcDonald, John F
dc.date.accessioned2013-06-12T15:12:51Z
dc.date.available2013-06-12T15:12:51Z
dc.date.issued2008-05-22
dc.identifier.citationMolecular Cancer. 2008 May 22;7(1):43
dc.identifier.urihttp://dx.doi.org/10.1186/1476-4598-7-43
dc.identifier.urihttp://hdl.handle.net/10724/19734
dc.description.abstractAbstract Background Previous findings have suggested that epigenetic-mediated HLA-G expression in tumor cells may be associated with resistance to host immunosurveillance. To explore the potential role of DNA methylation on HLA-G expression in ovarian cancer, we correlated differences in HLA-G expression with methylation changes within the HLA-G regulatory region in an ovarian cancer cell line treated with 5-aza-deoxycytidine (5-aza-dC) and in malignant and benign ovarian tumor samples and ovarian surface epithelial cells (OSE) isolated from patients with normal ovaries. Results A region containing an intact hypoxia response element (HRE) remained completely methylated in the cell line after treatment with 5-aza-dC and was completely methylated in all of the ovarian tumor (malignant and benign) samples examined, but only variably methylated in normal OSE samples. HLA-G expression was significantly increased in the 5-aza-dC treated cell line but no significant difference was detected between the tumor and OSE samples examined. Conclusion Since HRE is the binding site of a known repressor of HLA-G expression (HIF-1), we hypothesize that methylation of the region surrounding the HRE may help maintain the potential for expression of HLA-G in ovarian tumors. The fact that no correlation exists between methylation and HLA-G gene expression between ovarian tumor samples and OSE, suggests that changes in methylation may be necessary but not sufficient for HLA-G expression in ovarian cancer.
dc.titleEpigenetic Changes Within The Promoter Region of the HLA-G Gene in Ovarian Tumors
dc.typeJournal Article
dc.date.updated2013-06-07T18:48:10Z
dc.description.versionPeer Reviewed
dc.language.rfc3066en
dc.rights.holderLaura Menendez et al.; licensee BioMed Central Ltd.


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