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dc.contributor.authorNabyonga, Lydia
dc.contributor.authorKateete, David P
dc.contributor.authorKatabazi, Fred A
dc.contributor.authorOdong, Paul R
dc.contributor.authorWhalen, Christopher C
dc.contributor.authorDickman, Katherine R
dc.contributor.authorMoses, Joloba L
dc.date.accessioned2013-06-12T14:58:19Z
dc.date.available2013-06-12T14:58:19Z
dc.date.issued2011-08-11
dc.identifier.citationBMC Research Notes. 2011 Aug 11;4(1):280
dc.identifier.urihttp://dx.doi.org/10.1186/1756-0500-4-280
dc.identifier.urihttp://hdl.handle.net/10724/19630
dc.description.abstractAbstract Background Mycobacterial interspersed repetitive units - variable number of tandem repeats (MIRU-VNTR) genotyping is a powerful tool for unraveling clonally complex Mycobacterium tuberculosis (MTB) strains and detection of transmission patterns. Using MIRU-VNTR, MTB genotypes and their transmission patterns among patients with new and active pulmonary tuberculosis (PTB) in Kawempe municipality in Kampala, Uganda was determined. Results MIRU-VNTR genotyping was performed by PCR-amplification of 15 MTB-MIRU loci from 113 cultured specimens from 113 PTB patients (one culture sample per patient). To determine lineages, the genotypes were entered into the MIRU-VNTRplus database [http://www.miru-vntrplus.org/] as numerical codes corresponding to the number of alleles at each locus. Ten different lineages were obtained: Uganda II (40% of specimens), Uganda I (14%), LAM (6%), Delhi/CAS (3%), Haarlem (3%), Beijing (3%), Cameroon (3%), EAI (2%), TUR (2%) and S (1%). Uganda I and Uganda II were the most predominant genotypes. Genotypes for 29 isolates (26%) did not match any strain in the database and were considered unique. There was high diversity of MIRU-VNTR genotypes, with a total of 94 distinct patterns. Thirty four isolates grouped into 15 distinct clusters each with two to four isolates. Eight households had similar MTB strains for both index and contact cases, indicating possible transmission. Conclusion MIRU-VNTR genotyping revealed high MTB strain diversity with low clustering in Kawempe municipality. The technique has a high discriminatory power for genotyping MTB strains in Uganda.
dc.titleDetermination of circulating Mycobacterium tuberculosis Strains and Transmission Patterns among Pulmonary TB Patients in Kawempe Municipality, Uganda, using MIRU-VNTR
dc.typeJournal Article
dc.date.updated2013-06-07T14:54:23Z
dc.description.versionPeer Reviewed
dc.language.rfc3066en
dc.rights.holderLydia Nabyonga et al.; licensee BioMed Central Ltd.


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