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dc.contributor.authorGent, Jonathan I
dc.contributor.authorSchneider, Kevin L
dc.contributor.authorTopp, Christopher N
dc.contributor.authorRodriguez, Carmen
dc.contributor.authorPresting, Gernot G
dc.contributor.authorDawe, R Kelly
dc.date.accessioned2013-06-12T14:57:04Z
dc.date.available2013-06-12T14:57:04Z
dc.date.issued2011-02-25
dc.identifier.citationEpigenetics & Chromatin. 2011 Feb 25;4(1):3
dc.identifier.urihttp://dx.doi.org/10.1186/1756-8935-4-3
dc.identifier.urihttp://hdl.handle.net/10724/19620
dc.description.abstractAbstract Background Unique structural characteristics of centromere chromatin enable it to support assembly of the kinetochore and its associated tensions. The histone H3 variant CENH3 (centromeric histone H3) is viewed as the key element of centromere chromatin and its interaction with centromere DNA is epigenetic in that its localization to centromeres is not sequence-dependent. Results In order to investigate what influence the DNA sequence exerts on CENH3 chromatin structure, we examined CENH3 nucleosome footprints on maize centromere DNA. We found a predominant average nucleosome spacing pattern of roughly 190-bp intervals, which was also the dominant arrangement for nucleosomes genome-wide. For CENH3-containing nucleosomes, distinct modes of nucleosome positioning were evident within that general spacing constraint. Over arrays of the major ~156-bp centromeric satellite sequence (tandem repeat) CentC, nucleosomes were not positioned in register with CentC monomers but in conformity with a striking ~10-bp periodicity of AA/TT dimers within the sequence. In contrast, nucleosomes on a class of centromeric retrotransposon (CRM2) lacked a detectable AA/TT periodicity but exhibited tightly phased positioning. Conclusions These data support a model in which general chromatin factors independent of both DNA sequence and CENH3 enforce roughly uniform centromeric nucleosome spacing while allowing flexibility in the mode in which nucleosomes are positioned. In the case of tandem repeat DNA, the natural bending effects related to AA/TT periodicity produce an energetically-favourable arrangement consistent with conformationally rigid nucleosomes and stable chromatin at centromeres.
dc.titleDistinct influences of tandem repeats and retrotransposons on CENH3 nucleosome positioning
dc.typeJournal Article
dc.date.updated2013-06-07T16:40:36Z
dc.description.versionPeer Reviewed
dc.language.rfc3066en
dc.rights.holderJonathan I Gent et al.; licensee BioMed Central Ltd.


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