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dc.contributor.authorWang, Xiyin
dc.contributor.authorTorres, Manuel J
dc.contributor.authorPierce, Gary
dc.contributor.authorLemke, Cornelia
dc.contributor.authorNelson, Lisa K
dc.contributor.authorYuksel, Bayram
dc.contributor.authorBowers, John E
dc.contributor.authorMarler, Barry
dc.contributor.authorXiao, Yongli
dc.contributor.authorLin, Lifeng
dc.contributor.authorEpps, Ethan
dc.contributor.authorSarazen, Heidi
dc.contributor.authorRogers, Carl
dc.contributor.authorKarunakaran, Santhosh
dc.contributor.authorIngles, Jennifer
dc.contributor.authorGiattina, Emily
dc.contributor.authorMun, Jeong-Hwan
dc.contributor.authorSeol, Young-Joo
dc.contributor.authorPark, Beom-Seok
dc.contributor.authorAmasino, Richard M
dc.contributor.authorQuiros, Carlos F
dc.contributor.authorOsborn, Thomas C
dc.contributor.authorPires, J C
dc.contributor.authorTown, Christopher
dc.contributor.authorPaterson, Andrew H
dc.date.accessioned2013-06-12T14:53:59Z
dc.date.available2013-06-12T14:53:59Z
dc.date.issued2011-09-28
dc.identifier.citationBMC Genomics. 2011 Sep 28;12(1):470
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2164-12-470
dc.identifier.urihttp://hdl.handle.net/10724/19595
dc.description.abstractAbstract Background Evolution of the Brassica species has been recursively affected by polyploidy events, and comparison to their relative, Arabidopsis thaliana, provides means to explore their genomic complexity. Results A genome-wide physical map of a rapid-cycling strain of B. oleracea was constructed by integrating high-information-content fingerprinting (HICF) of Bacterial Artificial Chromosome (BAC) clones with hybridization to sequence-tagged probes. Using 2907 contigs of two or more BACs, we performed several lines of comparative genomic analysis. Interspecific DNA synteny is much better preserved in euchromatin than heterochromatin, showing the qualitative difference in evolution of these respective genomic domains. About 67% of contigs can be aligned to the Arabidopsis genome, with 96.5% corresponding to euchromatic regions, and 3.5% (shown to contain repetitive sequences) to pericentromeric regions. Overgo probe hybridization data showed that contigs aligned to Arabidopsis euchromatin contain ~80% of low-copy-number genes, while genes with high copy number are much more frequently associated with pericentromeric regions. We identified 39 interchromosomal breakpoints during the diversification of B. oleracea and Arabidopsis thaliana, a relatively high level of genomic change since their divergence. Comparison of the B. oleracea physical map with Arabidopsis and other available eudicot genomes showed appreciable 'shadowing' produced by more ancient polyploidies, resulting in a web of relatedness among contigs which increased genomic complexity. Conclusions A high-resolution genetically-anchored physical map sheds light on Brassica genome organization and advances positional cloning of specific genes, and may help to validate genome sequence assembly and alignment to chromosomes. All the physical mapping data is freely shared at a WebFPC site (http://lulu.pgml.uga.edu/fpc/WebAGCoL/brassica/WebFPC/; Temporarily password-protected: account: pgml; password: 123qwe123.
dc.titleA physical map of Brassica oleracea shows complexity of chromosomal changes following recursive paleopolyploidizations
dc.typeJournal Article
dc.date.updated2013-06-07T14:31:52Z
dc.description.versionPeer Reviewed
dc.language.rfc3066en
dc.rights.holderXiyin Wang et al.; licensee BioMed Central Ltd.


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