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dc.contributor.authorXia, Tian
dc.contributor.authorEiteman, Mark A
dc.contributor.authorAltman, Elliot
dc.date.accessioned2013-06-12T14:51:52Z
dc.date.available2013-06-12T14:51:52Z
dc.date.issued2012-06-12
dc.identifier.citationMicrobial Cell Factories. 2012 Jun 12;11(1):77
dc.identifier.urihttp://dx.doi.org/10.1186/1475-2859-11-77
dc.identifier.urihttp://hdl.handle.net/10724/19581
dc.description.abstractAbstract Background The efficient microbial utilization of lignocellulosic hydrolysates has remained challenging because this material is composed of multiple sugars and also contains growth inhibitors such as acetic acid (acetate). Using an engineered consortium of strains derived from Escherichia coli C and a synthetic medium containing acetate, glucose, xylose and arabinose, we report on both the microbial removal of acetate and the subsequent simultaneous utilization of the sugars. Results In a first stage, a strain unable to utilize glucose, xylose and arabinose (ALS1392, strain E. coli C ptsG manZ glk crr xylA araA) removed 3 g/L acetate within 30 hours. In a subsequent second stage, three E. coli strains (ALS1370, ALS1371, ALS1391), which are each engineered to utilize only one sugar, together simultaneously utilized glucose, xylose and arabinose. The effect of non-metabolizable sugars on the metabolism of the target sugar was minimal. Additionally the deletions necessary to prevent the consumption of one sugar only minimally affected the consumption of a desired sugar. For example, the crr deletion necessary to prevent glucose consumption reduced xylose and arabinose utilization by less than 15% compared to the wild-type. Similarly, the araA deletion used to exclude arabinose consumption did not affect xylose- and glucose-consumption. Conclusions Despite the modest reduction in the overall rate of sugar consumption due to the various deletions that were required to generate the consortium of strains, the approach constitutes a significant improvement in any single-organism approach to utilize sugars found in lignocellulosic hydrolysate in the presence of acetate.
dc.titleSimultaneous utilization of glucose, xylose and arabinose in the presence of acetate by a consortium of Escherichia coli strains
dc.typeJournal Article
dc.date.updated2013-06-07T13:40:49Z
dc.description.versionPeer Reviewed
dc.language.rfc3066en
dc.rights.holderTian Xia et al.; licensee BioMed Central Ltd.


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