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dc.contributor.authorLi, Mo
dc.contributor.authorBelozerov, Vladimir E
dc.contributor.authorCai, Haini N
dc.date.accessioned2013-06-12T14:39:10Z
dc.date.available2013-06-12T14:39:10Z
dc.date.issued2008-12-11
dc.identifier.citationBMC Molecular Biology. 2008 Dec 11;9(1):109
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2199-9-109
dc.identifier.urihttp://hdl.handle.net/10724/19522
dc.description.abstractAbstract Background Chromatin boundaries, also known as insulators, regulate gene activity by organizing active and repressive chromatin domains and modulate enhancer-promoter interactions. However, the mechanisms of boundary action are poorly understood, in part due to our limited knowledge about insulator proteins, and a shortage of standard assays by which diverse boundaries could be compared. Results We report here the development of an enhancer-blocking assay for studying insulator activity in Drosophila cultured cells. We show that the activities of diverse Drosophila insulators including suHw, SF1, SF1b, Fab7 and Fab8 are supported in these cells. We further show that double stranded RNA (dsRNA)-mediated knockdown of SuHw and dCTCF factors disrupts the enhancer-blocking function of suHw and Fab8, respectively, thereby establishing the effectiveness of using RNA interference in our cell-based assay for probing insulator function. Conclusion The novel boundary assay provides a quantitative and efficient method for analyzing insulator mechanism and can be further exploited in genome-wide RNAi screens for insulator components. It provides a useful tool that complements the transgenic and genetic approaches for studying this important class of regulatory elements.
dc.titleAnalysis of chromatin boundary activity in Drosophila cells
dc.typeJournal Article
dc.date.updated2013-06-07T18:43:38Z
dc.description.versionPeer Reviewed
dc.language.rfc3066en
dc.rights.holderMo Li et al.; licensee BioMed Central Ltd.


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